Dani Clos will drive for HRT during the first free practice session for his home grand prix in Spain this weekend.
It will be the first time that the Spaniard has got behind the wheel of the F112, with his previous experience with the team coming at last year's Abu Dhabi young driver test in the 2011 car.
Speaking about his opportunity, where he will take over Narain Karthikeyan's car, Clos said: "I'm delighted to have this opportunity. It's something I've fought for all my life; to make my debut in Formula 1 and, above all, to be able to do it at the Circuit de Catalunya which is something very special for me."
Clos's appearance for HRT alongside Pedro de la Rosa means that the Spanish team will take part in the session with two Spanish drivers for the first time in its history.
Team principal Luis Perez-Sala added: "Dani is a great driver and I have no doubt that he will do a good job. Besides, this debut also represents another step forward in our desire for HRT to serve as a platform for young drivers to make it into F1."