Audi opened the second day of testing at the top of the time sheets for the 59th Mobil 1 Twelve Hours of Sebring presented by Fresh From Florida. Audi Sport Team Joest's R15 prototype driven by Timo Bernard, Romain Dumas and Mike Rockenfeller posted a 1:48.450 (122.822mph) lap, 0.533sec off Audi's quickest time from Monday's test for the opening round of the 2011 American Le Mans Series presented by Tequila Patrón and Intercontinental Le Mans Cup.

Peugeot was also unable to improve on Monday's time and instead entered a 1:49.615 (121.516mph) at the hands of Franck Montagny, Pedro Lamy and Stephane Sarrazin, 1.165sec behind the No. 1 Audi R15 Plus. The second Audi R15 of Allan McNish, Dindo Capello and Tom Kristensen completed the top three in LMP1 with a lap of 1:50.080 (121.003mph).

The GT category remained incredibly tight with the top six cars separated by less than half a second. Corvette Racing's Corvette C6.R of Jan Magnussen, Oliver Gavin and Richard Westbrook led the way with a 2:02.043 (109.142mph), followed by BMW Motorsport's Augusto Farfus, Dirk Werner and Bill Auberlen. Their BMW M3 turned a 2:02.065 (109.122mph), but was quicker on Monday when the car topped the day with a 2:01.562 (109.574mph). Jaime Melo, Mika Salo and Toni Vilander were third in the Risi Competizione Ferrari F458 GTC, turning a 2:02.151 (109.045mph).

The Signatech Nissan-powered ORECA 03 continued to lead the LMP2 times as Franck Mailleux, Lucas Ordonez and Soheil Ayari entered a 1:55.627 (115.198mph). It was followed by the Oak Racing Pescarolo-Judd of Andrea Barlesi, Patrice Lagargue and Frederic DaRocha, with a 1:59.883 (111.108mph). Level 5's Scott Tucker, Christophe Bouchut and Joao Barbosa were third in their Lola-Honda coupe, turning a 2:01.510 (109.621mph).

CORE autosport held down the top spots in LMPC as Jon Bennett, Frankie Montecalvo and Ryan Dalziel turned a 1:55.852 (114.974mph), followed closely by the sister ORECA FLM09 of Gunnar Jeannette, Ricardo Gonzalez and Rudy Junco with a 1:56.364 (114.468mph). Not far behind was PR1/Mathiasen Motorsports' entry of Ken Dobson, Ryan Lewis and Henri Richard, which turned in a 1:56.545 (114.291mph).

Patrick Bornhauser, Julien Canal and Michele Rugolo were the GTE-AM leaders with a 2:04.631 (106.875mph) in the Larbre Competition Chevrolet Corvette C6.R followed by Pierre Ehert, Shaun Lynn and Roger Willis at a 2:07.561 (104.421mph) in the CRS Racing Ferrari F430.

Bill Sweedler, Leh Keen and Brian Wong were the early GTC leaders on Tuesday after turning a 2:06.645 (105.176mph) in their Porsche 911 GT3 Cup entry. GMG Racing's Bret Curtis, Jan Seyffarth and James Sofronas were second quickest with a 2:07.374 (104.574mph). TRG's Porsche of Dion von Moltke, Peter Ludwig and Jim Norman completed the top three with a 2:07.698 (104.309mph).